Intended transcriptional silencing with siRNA results in gene repression through sequence-specific off-targeting
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چکیده
منابع مشابه
Intended transcriptional silencing with siRNA results in gene repression through sequence-specific off-targeting.
Transcriptional gene silencing has been reported with siRNA targeting the promoter region of genes. We tested several siRNAs directed against the human VEGF promoter. Of these, siVFp(-992) exhibited > or =50% suppression of VEGF production in two human cell lines. To determine the specificity of this siRNA-mediated suppression, plasmids were prepared to express a luciferase reporter under the c...
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ژورنال
عنوان ژورنال: RNA
سال: 2009
ISSN: 1355-8382
DOI: 10.1261/rna.1808510